pRS422 (ATCC® 87479)

Clone Type: Vector YE-type (episomal) shuttle vector  /  Depositors: JD Boeke

Permits and Restrictions

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Designations pRS422
GenBank Number

U93719

Depositors JD Boeke
Applications
vector permitting visual detection of recombinants phosphoribosylaminoimidazole succinocarboxamide synthetase SAICAR synthetase, phosphoribosylaminoimidazole carboxylase
Vector
Intact vector size: 6.868
Type of vector: phagemid
Cloning sites: SacI SacII EagI NotI SpeI BamHI SmaI PstI EcoRI ClaI SalI XhoI ApaI KpnI
Polylinker sites: SacI BstXI SacII EagI NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI ApaI KpnI
Construction: pRS402, pRS421
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): ADE2, →, 575-2290
replicon: f1, ←, 2495-2951
promoter for in vitro transcription: T7, →, 3121-3140
MCS: KpnI...SacI, →, 3148-3250
promoter for in vitro transcription: T3, ←, 3267-3286
coding sequence: lacZ', ←, 2952-3311
promoter: lac, ←, 3356-3384
replicon: pMB1, 3710-3710
marker(s): ampR, ←, 4468-5328
replicon: 2 micron, 5460-6801
Vector: pRS422 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pRS402, pRS421
Marker(s): ampR,ADE2
Construct size (kb): 6.868
Features: marker(s): ADE2
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
coding sequence: lacZ'
Insert
Target Gene: , phosphoribosylaminoimidazole succinocarboxamide synthetase
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products
Component of: ATCC 87480
Component of: ATCC 87530
Component of: ATCC 87535
Biosafety Level 1
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.8; BglII--4.6, 2.2; EcoRI--6.8.
ade2 phenotype produces red colonies when grown on adenine containing media.
This vector is useful for gene knockout experiments in hosts with a non-revertable ade2 auxotrophic marker gene mutation.
References

Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801

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Attachments Vector map for 87479