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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 6.2329998016357420 Vector: pRS421 (phagemid) Promoters: Promoter for in vitro transcription T7 Construction: pRS401, YEp24 Marker(s):ampR,MET15 Construct size (kb): 6.232999801635742 Features: insert detection: lacZ'
marker(s): MET15
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
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Applications
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YE-type (episomal) shuttle vector vector permitting visual detection of recombinants O-acetylhomoserine sulfhydrylase
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Comments
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Restriction digests of the clone give the following sizes (kb): BamHI--6.2; EcoRI--1.7, 4.5; XbaI--3.2, 2.0, 1.0. met15 phenotype produces brown colonies when grown on Pb containing media. MET15, MET17 and MET25 are synonymous. This vector is useful for gene knockout experiments in hosts with a non-revertable met15 auxotrophic marker gene mutation.
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References
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Cost GJ, Boeke JD. A useful colony colour phenotype associated with the yeast selectable/counter-selectable marker MET15. Yeast 12: 939-941, 1996. PubMed: 8873447
Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801
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Related Products
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component of:ATCC 87476
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Shipping Information
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Frozen glycerol stock of Escherichia coli containing the plasmid.
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