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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Host
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Distribution host: Escherichia coli DH5alpha
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Vector Information
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Size (kb): 5.314 DESCRIPTION OF VECTOR:
Intact vector size: 5.314
Type of vector: plasmid
Cloning sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV PstI SmaI BamHI SpeI
XbaI BstXI SacII SacI
Polylinker sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI
SpeI XbaI BstXI SacII SacI
Construction: pBBR1MCS, tmpR
Host range: broad host range; Burkholderia pseudomallei; Burkholderia
cepacia; Escherichia coli
Features (with orientation and position when available):
coding sequence: MOB, <-, 5148-799
coding sequence: REP, ->, 1793-2455
MCS: SacI...KpnI, ->, 3220-3322
marker(s): tmpR, ->, 4387-4623 Vector: pBBR1Tp (plasmid) Construction: pBBR1MCS, tmpR Marker(s):tmpR Construct size (kb): 5.314 Features: marker(s): tmpR
MCS: SacI...KpnI
coding sequence: MOB
coding sequence: REP
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Applications
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cloning vector mobilizable vector vector permitting visual detection of recombinants
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Comments
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Restriction digests of the clone give the following sizes (kb): PstI--5.4; EcoRI--4.0, 0.64, 0.8; PvuII--2.4, 1.35, 1.1, 0.4 Plasmid is compatible with multiple replicons and can be transferred to other gram-negative bacteria by conjugation when the RK2 transfer functions are provided in trans. Broad host range cloning vector allowing blue/white color detection of recominants and carrying the trimethoprim resistance gene as a selectable marker.
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References
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DeShazer D, Woods DE. Broad-host-range cloning and cassette vectors based on the R388 trimethoprim resistance gene. BioTechniques 20: 762-764, 1996. PubMed: 8723912
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Shipping Information
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Distributed: freeze-dried
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