pPW121 (ATCC® 87444)

Applications: vector permitting positive selection for inserts  /  Depositors: F Puta

Permits and Restrictions

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Designations pPW121
Depositors F Puta
Biosafety Level 1
Vector Information
Size (kb): 4.3860001564025880
Vector: pPW121 (plasmid)
Promoters: Promoter lambda PR
Construction: pUN121
Marker(s):ampR,tetR
Construct size (kb): 4.386000156402588
Features: marker(s): ampR
marker(s): tetR
operator: lac
promoter: lambda PR
replicon: pMB1
repressor gene: cI
Applications
vector permitting positive selection for inserts
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; HindIII--4.3; BamHI--4.3.
Postive selection cloning vector which confers tetR (recommended concentration 10 ug/ml) only when an insert has been successfully cloned into the cI repressor gene.
Plasmid was derived from pUN121 by introduction of a BglII site into the cI gene. Plasmid pUN121 was derived from pTR262 by addition of EcoRI and SmaI/XmaI sites in the cI gene.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Puta F, Wambutt R. Construction of a new Escherichia coli-Saccharomyces cerevisiae shuttle plasmid cloning vector allowing positive selection for cloned fragments. Folia Microbiol. 37: 193-198, 1992. PubMed: 1505881

Nilsson B, et al. An improved positive selection plasmid vector constructed by oligonucleotide mediated mutagenesis. Nucleic Acids Res. 11: 8019-8030, 1983. PubMed: 6316281

Frantisek Puta, personal communication

Shipped freeze-dried
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