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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Species
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Cryptosporidium parvum Tyzzer
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Vector
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DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references:
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Vector
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DNA: genomic Digest: Complete Insert size range: 0.2-5.0 Kb Coverage: Clones for "complete" coverage: 15000.0
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Shipping Information
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Distributed: frozen (volume: 0.4 mL)
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Comments
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The KSU-1 isolate of cryptosporidium parvum was isolated from the feces of naturally infected calf (Bos taurus) in 1987. The isolate was passaged 6-10 times per year by oral inoculation of goats, mice and/or calves prior to library construction in 1992. Approximately 1% of the recombinants in this library contain two ligated EcoRI inserts.
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References
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Khramtsov NV, et al. Cloning and analysis of a Cryptosporidium parvum gene encoding a protein with homology to cytoplasmic form hsp70. J. Eukaryot. Microbiol. 42: 416-422, 1995. PubMed: 7620467
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