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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Origin
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lymphoblast cell line GM03714 (46, XX)
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Applications
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Microdissection library derived from human chromosome 2. Can be used for chromosome painting and for isolating region specific probes.
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Vector
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Tissue: lymphoblast cell line GM03714 (46, XX) Chromosome: 2p11-q11 DNA: genomic Digest: Complete Insert size range: 50-600 bp
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Comments
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Microdissection library derived from human chromosome 2. Can be used for chromosome painting and for isolating region specific probes. The library covers the region 2p11.1-2q11.1. Approximately 50% of the clones contain repetitive sequences. Of 46 single copy clones tested, 37 (80%) hybridized to chromosome 2. Constructed by microdissecting fragments from 20 chromosomes, treating with proteinase K, extracting with phenol, ligating to an MboI linker-adaptor, amplifying by PCR, digesting with MboI, and ligating to pUC19.
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References
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Kao FT, et al. Complete set of eleven region-specific microdissection libraries for human chromosome 2. Somatic Cell Mol. Genet. 22: 57-66, 1996. PubMed: 8643994
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