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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 4.9000000953674320 Vector: pKKM13(+) (phagemid) Promoters: Promoter for expression tac Marker(s):ampR Construct size (kb): 4.900000095367432 Features: marker(s): ampR
promoter for expression: tac
replicon: M13
replicon: pMB1
MCS: EcoRI...HindIII
transcription terminator: T1
transcription terminator: T2
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Applications
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expression vector vector permitting production of single-stranded DNA vector useful for site-directed mutagenesis
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Comments
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Restriction digests of the clone give the following sizes (kb): EcoRI--4.8; BamHI--4.6, 0.2; XbaI--4.8. Expression vector useful for mutagenesis of cloned inserts. Single stranded DNA obtained after superinfection with a suitable M13 helper phage gives the sense strand with respect to the tac promoter.
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References
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Nock S, et al. One-vector system for mutagenesis and over-expression of cloned genes. BioTechniques 18: 608, 1995. PubMed: 7598889
Roland Kreutzer, personal communication
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