pKKM13(+) (ATCC® 87403)

Applications: expression vectorvector permitting production of single-stranded DNAvector useful for site-directed mutagenesis  /  Depositors: R Kreutzer

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Designations pKKM13(+)
Depositors R Kreutzer
Biosafety Level 1
Vector Information
Size (kb): 4.9000000953674320
Vector: pKKM13(+) (phagemid)
Promoters: Promoter for expression tac
Marker(s):ampR
Construct size (kb): 4.900000095367432
Features: marker(s): ampR
promoter for expression: tac
replicon: M13
replicon: pMB1
MCS: EcoRI...HindIII
transcription terminator: T1
transcription terminator: T2
Applications
expression vector
vector permitting production of single-stranded DNA
vector useful for site-directed mutagenesis
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.8; BamHI--4.6, 0.2; XbaI--4.8.
Expression vector useful for mutagenesis of cloned inserts.
Single stranded DNA obtained after superinfection with a suitable M13 helper phage gives the sense strand with respect to the tac promoter.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Nock S, et al. One-vector system for mutagenesis and over-expression of cloned genes. BioTechniques 18: 608, 1995. PubMed: 7598889

Roland Kreutzer, personal communication

Shipped frozen
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