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pBAD18-Kan (ATCC^® 87397^™)

Applications: expression vectorproduces protein arabinose regulatorvector containing primer sites useful for sequencing / Depositors: L Guzman, J Beckwith

pBAD18-Kan ATCC^® 87397^™

freeze-dried

For-Profit: $190.00 Non-Profit: $190.00

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Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Designations	pBAD18-Kan
Depositors	L Guzman, J Beckwith
Biosafety Level	1
Vector Information	Size (kb): 5.4369997978210450 DESCRIPTION OF VECTOR: Intact vector size: 5.437 Type of vector: phagemid Cloning sites: NheI EcoRI SacI KpnI XbaI SalI PstI SphI Polylinker sites: NheI EcoRI SacI KpnI SmaI BamHI XbaI SalI AccI PstI SphI HindIII Construction: pBAD18, kanR (pUC4K) Host range: Escherichia coli Features (with orientation and position when available): regulator: araC, <-, 3346-4224 operator: O2, 4253-4270 promoter: araC, <-, 4375-4403 operator: O1, 4411-4432 other: CAP site, 4454-4467 operator: I2 + I1, 4463-4501 promoter for expression: arabinose BAD, ->, 4500-4527 MCS: NheI...HindIII, ->, 4550-4612 transcription terminator: rrnB T1 + T2, ->, 4613-5038 marker(s): kanR, ->, 123-938 replicon: M13, 1417-1875 replicon: pMB1, 1881-2578 Vector: pBAD18-Kan (phagemid) Promoters: Promoter araC Construction: pBAD18, kanR (pUC4K) Marker(s):kanR,araC Construct size (kb): 5.436999797821045 Features: marker(s): kanR operator: I2 + I1 operator: O1 operator: O2 other: CAP site promoter: araC promoter for expression: arabinose BAD regulator: araC replicon: M13 replicon: pMB1 MCS: NheI...HindIII transcription terminator: rrnB T1 + T2
Applications	expression vector produces protein arabinose regulator vector containing primer sites useful for sequencing
Comments	Restriction digests of the clone give the following sizes (kb): EcoRI--5.4; HindIII--3.9, 1.5; PstI--5.4. Cultures should be grown in minimal media for more reproducible induction of expression. Expression is induced in glycerol-containing media by addition of arabinose. Expression is repressed by addition of glucose or other catabolites. One of several tightly controlled expression vectors (ATCC 87393-87402) regulated by the arabinose operon. The vectors differ in replicon, antibiotic resistance marker, multiple cloning site and mechanism of initiation of translation. The following primers can be used for sequencing of cloned inserts: 5' primer (27 - 8 bp upstream of the NheI site) 5'-CTGTTTCTCCATACCCGTT-3'; and one of two 3' primers: 3' primer 1 (2 - 19 bp downstream of the HindIII site) 5'-CTCATCCGCCAAAACAG-3'; 3' primer 2 (17 - 33 bp downstream of the HindIII site) 5'-GGCTGAAAATCTTCTCT-3'. Cloned inserts must provide a translation initiation sequence (ATG) and ribosome binding site for expression. Plasmid contains bla (ampR) sequences surrounding the kanR gene which could promoter recombination if this plasmid is used in combination with other compatible ampR plasmids. Recombination can be avoided by the use of recA host strains, or it can be used to advantage to intentionally exchange markers among plasmids.
Growth Conditions	Temperature: 37.0°C
References	Guzman LM, et al. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 177: 4121-4130, 1995. PubMed: 7608087
Shipped	FD
Shipping Information	Distributed: freeze-dried

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Product Sheet	Product Sheet
Attachments	Vector Map