p416 GPD (ATCC® 87360)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

Permits and Restrictions

View Permits

Designations p416 GPD
Depositors M Funk
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 5.8039999008178710
Intact vector size: 5.804
Type of vector: plasmid
Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI BamHI SpeI XbaI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS416
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
replicon: CEN6/ARSH4, ->
marker(s): URA3, ->
terminator: CYC1, ->
promoter for expression: GPD, <-
marker(s): ampR, <-
Vector: p416 GPD (plasmid)
Promoters: Promoter for expression GPD
Construction: pRS416
Construct size (kb): 5.803999900817871
Features: marker(s): URA3
marker(s): ampR
promoter for expression: GPD
replicon: CEN6/ARSH4
terminator: CYC1
YX-type (expression) shuttle vector
vector with low copy number
Restriction digests of the clone give the following sizes (kb): SstI/XbaI--5.13, 0.68; EcoRI--5.8, BamHI--5.8.
Low copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

Related Products
component of:ATCC 87669
Shipped frozen
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map