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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Origin
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lymphoblast cell line GM03714 (46, XX)
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Applications
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Colonies of different sizes may be observed.
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Vector
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Tissue: lymphoblast cell line GM03714 (46, XX) Chromosome: 2q31-q32 DNA: genomic Digest: Complete Insert size range: 50-800 bp Coverage: Clones for "complete" coverage: 630000.0
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Comments
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Colonies of different sizes may be observed. Region specific library constructed by microdissection of metaphase chromosomes, complete digestion with MboI, followed by PCR amplification and subcloning of amplification products. Approximately 70% of unique sequences were derived from chromosome 2, as determined by hybridization. Half of all the clones contain repetitive sequence which should be human and chromosome 2 specific. The library is estimated to be 85% pure. Approximately half of the clones in the library contain unique or low-copy number inserts. The mean insert size is 190 bp.
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References
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Yu J, et al. Three region-specific microdissection libraries for the long arm of human chromosome 2, regions q33-q35, q31-q32 and q23-q24. Somatic Cell Mol. Genet. 21: 335-343, 1995. PubMed: 8619130
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