2Q2 (ATCC® 87308)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: F Kao

Designations 2Q2
Species Homo sapiens, human
Depositors F Kao
Origin
lymphoblast cell line GM03714 (46, XX)
Applications
Colonies of different sizes may be observed.
Vector
Tissue: lymphoblast cell line GM03714 (46, XX)
Chromosome: 2q33-q35
DNA: genomic
Digest: Complete
Insert size range: 50-800 bp
Coverage: Clones for "complete" coverage: 850000.0
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Comments
Colonies of different sizes may be observed.
Region specific library constructed by microdissection of metaphase chromosomes, complete digestion with MboI, followed by PCR amplification and subcloning of amplification products.
Approximately 94% of unique sequences were derived from chromosome 2, as determined by hybridization. Half of the clones contain repetitive sequences and all repetitive sequences are human and chromosome 2 specific.
The library is estimated to be 97% pure.
Approximately half of the clones in the library contain unique or low-copy number inserts.
The mean insert size is 130 bp.
References

Yu J, et al. Three region-specific microdissection libraries for the long arm of human chromosome 2, regions q33-q35, q31-q32 and q23-q24. Somatic Cell Mol. Genet. 21: 335-343, 1995. PubMed: 8619130

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