|
Permits
|
These permits may be required for shipping this product:
-
Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
|
|
Designations
|
lambdaACT2 - human prostate cDNA
|
|
Vector
|
DESCRIPTION OF VECTOR COMPONENT: Name of vector: lambdaACTII Intact vector size: 42.300 Type of vector: phage Vector end: EcoRI Vector end: XhoI Cloning sites: NcoI SfiI SmaI BamHI EcoRI XhoI BglII Polylinker sites: NdeI NcoI SfiI SmaI BamHI EcoRI XhoI BglII Construction: lambdaYES, pACT2 ( ATCC 87006) Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR, LEU2 promoter: ADC1 replicon: pMB1, 2 micron, lambda repressor gene: cI857 terminator: ADC1 Cross references: |
|
Vector
|
Tissue: prostate DNA: cDNA Insert size range: 0.7 - 7 kb
|
|
Shipping Information
|
Distributed: frozen bacteria-free lysate (volume: 0.4 mL)
|
|
Comments
|
In yeast, the ADH promoter drives expression of a fusion protein consisting of the GAL4 activation domain, a hemagglutinin tag, and the sequences directed by the insert.
|
|
References
|
Durfee T, et al. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555-569, 1993. PubMed: 8384581
Stephen J Elledge, personal communication
|