lambdaACT2 - human prostate cDNA (ATCC® 87296)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: SJ Elledge

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Designations lambdaACT2 - human prostate cDNA
Species Homo sapiens, human
Depositors SJ Elledge
Origin
prostate
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdaACTII
Intact vector size: 42.300
Type of vector: phage
Vector end: EcoRI
Vector end: XhoI
Cloning sites: NcoI SfiI SmaI BamHI EcoRI XhoI BglII
Polylinker sites: NdeI NcoI SfiI SmaI BamHI EcoRI XhoI BglII
Construction: lambdaYES, pACT2 (ATCC 87006)
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, LEU2
promoter: ADC1
replicon: pMB1, 2 micron, lambda
repressor gene: cI857
terminator: ADC1
Cross references:
Vector
Tissue: prostate
DNA: cDNA
Insert size range: 0.7 - 7 kb
Biosafety Level 1
Shipping Information Distributed: frozen bacteria-free lysate (volume: 0.4 mL)
Comments
In yeast, the ADH promoter drives expression of a fusion protein consisting of the GAL4 activation domain, a hemagglutinin tag, and the sequences directed by the insert.
References

Durfee T, et al. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555-569, 1993. PubMed: 8384581

Stephen J Elledge, personal communication

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