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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Designations
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lambdaACT2 - human breast cDNA library
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Vector
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Genome: human
Tissue: breast
Type of DNA: cDNA
Insert size: 0.7 – 7 kb
Number of independent recombinants: 1.0 x 108
Non-recombinants (%): 5.0
Insert 5’ end:
Insert 3’ end: XhoI adapters
Titer: 9.5 x 108 pfu/mL
Vector: lambdaACTII
Intact vector size (kb): 42.300
Type of vector: phage
Features:
Cloning sites: NcoI SfiI SmaI BamHI EcoRI XhoI BglII
Promoter: ADCI
Replicon: pMB1, 2 micron, lambda
Repressor gene: cI857
Terminator: ADC1
Construction: lambdaYES, pACT2 (ATCC#87006)
Vector ends: EcoRI, XhoI
Markers: ampR, LEU2
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Comments
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In yeast, the ADH promoter drives expression of a fusion protein consisting of the GAL4 activation domain, a hemagglutinin tag, and the sequences directed by the insert.
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References
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Durfee T, et al. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555-569, 1993. PubMed: 8384581
Stephen J Elledge, personal communication
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