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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Designations
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lambdaACT - Schizosaccharomyces pombe cDNA
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Species
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Schizosaccharomyces pombe Lindner, teleomorph, fission yeast
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Vector
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Marker(s):LEU2,ampR DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdaACT
Intact vector size: 42.400
Type of vector: phage
Vector end: XhoI
Vector end: XhoI
Cloning sites: XhoI
Polylinker sites: BglII EcoRI BamHI XhoI BglII
Construction: lambdaYES, pSE1107
Host range: Escherichia coli
Features (with orientation and position when available):
other: left arm (19.6 kb), ->
restriction site: NotI, ->
other: lox, ->
marker(s): LEU2, <-
replicon: 2 micron
marker(s): ampR, ->
replicon: pMB1
promoter: ADH, ->
other: GAL4 activation domain, ->
MCS: BglII/EcoRI...XhoI/BglII, ->
terminator: ADH, ->
other: lox, ->
restriction site: NotI
other: right arm (15.2 kb, with cI857)
Cross references:
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Vector
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DNA: cDNA Cloning site: XhoI Insert size range: 0.7 - 7 kb Insert ends: XhoI adaptors Number of independent recombinants: 1 x 10^8 Non-recombinants (%): 5
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Shipping Information
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Distributed: frozen bacteria-free lysate (volume: 0.4 mL)
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References
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Durfee T, et al. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555-569, 1993. PubMed: 8384581
recommended for amplification
recommended for cre-lox based conversion to plasmid
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