|
Permits
|
These permits may be required for shipping this product:
-
Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
|
|
Designations
|
lambdaHA - Saccharomyces cerevisiae cDNA
|
|
Species
|
Saccharomyces cerevisiae Meyen ex E.C. Hansen
|
|
Vector
|
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdaHA
Intact vector size: 42.600
Type of vector: phage
Vector end: XhoI
Vector end: XhoI
Cloning sites: XhoI, EcoRI
Polylinker sites: EcoRI XhoI EcoRI
Cloning capacity: 0 - 8.0 kb
Construction: lambdaYES-R
Host range: Escherichia coli
Features (with orientation and position when available):
other: left arm (19.6 kb), ->
other: lox, ->
replicon: ARS1
replicon: pMB1
marker(s): ampR, <-
promoter for expression: GAL1, ->
epitope tag: hemagglutinin (HA), ->
MCS: XhoI EcoRI, ->
promoter for expression: lac, <-
transcription terminator: HIS3, ->
marker(s): URA3, ->
centromere: CEN4
other: lox, ->
other: right arm (15.2 kb, with cI857)
Cross references:
|
|
Vector
|
DNA: cDNA Insert size range: 0.7 - 7 kb
|
|
Shipping Information
|
Distributed: frozen bacteria-free lysate (volume: 0.4 mL)
|
|
Comments
|
Inserts in the correct frame will be expressed with a hemagglutinin epitope tag at the amino terminus. The vector is derived from lambdaYES-R. A sequence providing translation initiatin signals and encoding the epitope tag was inserted between the GAL1 promoter and the cloning site.
|
|
Classification
|
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
|
|
References
|
Stephen J Elledge, personal communication
|