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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Designations
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lambdaLEU - human B cell cDNA
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Origin
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B lymphocyte (EBV transformed)
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Vector
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DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdaLEU
Intact vector size: 42.000
Type of vector: phage
Vector end: XhoI
Vector end: XhoI
Cloning sites: XhoI
Polylinker sites: EcoRI XhoI EcoRI
Cloning capacity: 0 - 8.4 kb
Construction: lambdaYES-R, pLEU
Host range: Escherichia coli
Features (with orientation and position when available):
other: left arm (19.6 kb), ->
other: lox, ->
replicon: ARS1
replicon: pMB1
marker(s): ampR, <-
promoter for expression: GAL1, ->
MCS: EcoRI XhoI EcoRI, ->
promoter for expression: lac, <-
transcription terminator: HIS3, ->
marker(s): LEU2, ->
centromere: CEN4
other: right arm (15.2 kb, with cI857)
Cross references:
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Vector
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Tissue: B lymphocyte (EBV transformed) DNA: cDNA Insert size range: 0.7 - 7 kb Insert ends: XhoI adaptors Insert site: XhoI Number of independent recombinants: 1 x 10^8 Non-recombinants (%): 5
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Shipping Information
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Distributed: frozen bacteria-free lysate (volume: 0.4 mL)
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