lambdaYES - Drosophila third instar cDNA (ATCC® 87274)

Organism: Drosophila melanogaster, fruit fly  /  Clone Type: Library  /  Depositors: SJ Elledge

Permits and Restrictions

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Designations lambdaYES - Drosophila third instar cDNA
Species Drosophila melanogaster, fruit fly
Depositors SJ Elledge
Origin
larva, third instar
Applications
To convert bacteriophage lambda clones to plasmid via the cre-lox recombination system, use of BNN132 (ATCC 47059) is recommended.
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdaYES-R
Intact vector size: 42.600
Type of vector: phage
Vector end: XhoI
Vector end: XhoI
Cloning sites: XhoI, EcoRI
Polylinker sites: EcoRI XhoI EcoRI
Construction: lambdagt6; pSE936
Host range: Escherichia coli
Features (with orientation and position when available):
other: left arm (19.6 kb), ->
other: lox, ->
replicon: ARS1
replicon: pMB1
marker(s): ampR, <-
promoter for expression: GAL1, ->
MCS: XhoI EcoRI, ->
promoter for expression: lac, <-
transcription terminator: HIS3, ->
marker(s): URA3, ->
centromere: CEN4
other: lox, ->
other: right arm (15.2 kb, with cI857)
Cross references:
Vector
Tissue: larva, third instar
DNA: cDNA
Insert size range: 0.7 - 7 kb
Biosafety Level 1
Shipping Information Distributed: frozen bacteria-free lysate (volume: 0.4 mL)
Comments
To convert bacteriophage lambda clones to plasmid via the cre-lox recombination system, use of BNN132 (ATCC 47059) is recommended.
References

Elledge SJ, et al. lambdaYES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc. Natl. Acad. Sci. USA 88: 1731-1735, 1991. PubMed: 1848010

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