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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Designations
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lambdaYES - human B cell cDNA
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Origin
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B lymphocyte (EBV transformed)
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Applications
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To convert bacteriophage lambda clones to plasmid via the cre-lox recombination system, the use of host strain E. coli BNN 132 ( ATCC 47059) is recommended. |
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Vector
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DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdaYES-R
Intact vector size: 42.600
Type of vector: phage
Vector end: XhoI
Vector end: XhoI
Cloning sites: XhoI, EcoRI
Polylinker sites: EcoRI XhoI EcoRI
Construction: lambdagt6; pSE936
Host range: Escherichia coli
Features (with orientation and position when available):
other: left arm (19.6 kb), ->
other: lox, ->
replicon: ARS1
replicon: pMB1
marker(s): ampR, <-
promoter for expression: GAL1, ->
MCS: XhoI EcoRI, ->
promoter for expression: lac, <-
transcription terminator: HIS3, ->
marker(s): URA3, ->
centromere: CEN4
other: lox, ->
other: right arm (15.2 kb, with cI857)
Cross references:
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Vector
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Tissue: B lymphocyte (EBV transformed) DNA: cDNA Insert size range: 0.7 - 7 kb Insert size range (kb): 0.7 - 7 Insert ends: XhoI adaptors Insert site: XhoI Number of independent recombinants: 1 x 10^8 Non-recombinants (%): 5
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Shipping Information
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Distributed: frozen bacteria-free lysate (volume: 0.4 mL)
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Comments
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To convert bacteriophage lambda clones to plasmid via the cre-lox recombination system, the use of host strain E. coli BNN 132 ( ATCC 47059) is recommended. |
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References
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Elledge SJ, et al. lambdaYES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc. Natl. Acad. Sci. USA 88: 1731-1735, 1991. PubMed: 1848010
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