human embryonic brain, 67 day - 1 (ATCC® 87249)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: DM Kurnit

Permits and Restrictions
Permits and Restrictions

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Designations human embryonic brain, 67 day - 1
Species Homo sapiens, human
Depositors DM Kurnit
Origin
embryonic brain, 67 day
Applications
This host is strongly recommended for screening and amplification of the library.
The host DA1061 (ATCC 47088) suppresses the formation of large non-lambda phage plaques.
Vector
Vector : Lambda ZAPII
Vector type : phage
Intact vector size (kb) : 40.820
Construction : pBluescript SK-
Features : (with orientation and position, when available) :
Insert detection : lacZ'
Marker : ampR
Promoter : lac, T3, T7
Replicon : pMB1, f1, lambda
Vector
Tissue: embryonic brain, 67 day
Type of insert: cDNA
Digest: Complete
Insert size range: 0.3 - 0.5 kb
Coverage: Clones for "complete" coverage: 1000000.0
Vector : lambda ZAPII
Vector ends : Modification : EcoRI adapters
Number of independent recombinants : 8.0 x 105
Nonrecombinants (%) : 34.0
Titer : 8.0 x 107 pfu/ml
Biosafety Level 1
Shipping Information Frozen bacteria-free phage lysate.
Comments
The host DA1061 (ATCC 47088) suppresses the formation of large non-lambda phage plaques. This host is strongly recommended for screening and amplification of the library.
References

Buraczynska MJ, et al. Construction of human embryonic cDNA libraries: HD, PKD1 and BRCA1 are transcribed widely during embryogenesis. Cytogenet. Cell Genet. 71: 197-202, 1995. PubMed: 7656596

David M Kurnit, personal communication

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Product Sheet
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