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Designations
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human embryonic brain, 67 day - 1
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Applications
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This host is strongly recommended for screening and amplification of the library. The host DA1061 ( ATCC 47088) suppresses the formation of large non-lambda phage plaques. |
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Vector
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Vector : Lambda ZAPII Vector type : phage Intact vector size (kb) : 40.820 Construction : pBluescript SK- Features : (with orientation and position, when available) :
Insert detection : lacZ'
Marker : ampR
Promoter : lac, T3, T7
Replicon : pMB1, f1, lambda
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Vector
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Tissue: embryonic brain, 67 day Type of insert: cDNA Digest: Complete Insert size range: 0.3 - 0.5 kb Coverage: Clones for "complete" coverage: 1000000.0 Vector : lambda ZAPII Vector ends : Modification : EcoRI adapters Number of independent recombinants : 8.0 x 105 Nonrecombinants (%) : 34.0 Titer : 8.0 x 107 pfu/ml
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Shipping Information
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Frozen bacteria-free phage lysate.
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Comments
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The host DA1061 ( ATCC 47088) suppresses the formation of large non-lambda phage plaques. This host is strongly recommended for screening and amplification of the library. |
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References
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Buraczynska MJ, et al. Construction of human embryonic cDNA libraries: HD, PKD1 and BRCA1 are transcribed widely during embryogenesis. Cytogenet. Cell Genet. 71: 197-202, 1995. PubMed: 7656596
David M Kurnit, personal communication
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