human embryonic heart, 67d (ATCC® 87234)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: DM Kurnit

Permits and Restrictions
Permits and Restrictions

View Permits

Designations human embryonic heart, 67d
Species Homo sapiens, human
Depositors DM Kurnit
Origin
embryonic heart, 67 day
Applications
This host is strongly recommended for screening and amplification of the library.
The host DA1061 (ATCC 47088) suppresses the formation of large non-lambda phage plaques.
Vector
Vector : lambda-ZAPII

Vector type : phage

Intact vector size (kb) : 40.18

Features : (with orientation and position, when available) :
Insert detection : lacZ?
Marker : ampR
Promoter for in vitro transcription : T3, T7
Replicon : pMB1, f1, lambda

Vector
Tissue: embryonic heart, 67 day
DNA: cDNA
Digest: Complete
Insert size range: 0.3 - 0.5 kb
Coverage: Clones for "complete" coverage: 1000000.0
Biosafety Level 1
Comments
The host DA1061 (ATCC 47088) suppresses the formation of large non-lambda phage plaques. This host is strongly recommended for screening and amplification of the library.
References

Buraczynska MJ, et al. Construction of human embryonic cDNA libraries: HD, PKD1 and BRCA1 are transcribed widely during embryogenesis. Cytogenet. Cell Genet. 71: 197-202, 1995. PubMed: 7656596

David M Kurnit, personal communication

Product Sheet
Product Sheet
Product Sheet
Attachments Product Information Sheet