Size (kb): 4.4429998397827150
Vector: pOSEX5A (plasmid)
Promoters: Promoter for expression proU
Construct size (kb): 4.442999839782715
Features: initiation codon: ATG
promoter for expression: proU
replicon: rop (copy number control)
transcription terminator: rrnB T1
transcription terminator: rrnB T2
coding sequence: proV 5' sequence
vector permitting construction of fusion proteins
Restriction digests of the clone give the following sizes (kb): BamHI--4.4; HindIII--4.4; PvuII--4.4.
Expression can be induced in cells grown in low osmolarity media by the addition of sodium chloride.
Expression vector allowing osmotically controlled expression of cloned inserts directed by the E. coli proU promoter.
Absence of a stop codon between the proV' sequence and the ATG start codon allows production of a fusion protein between ProV and the cloned insert. Insertion of a stop codon at this junction may reduce the yield of recombinant protein.
This material is being provided with the explicit understanding that it not be used for commercial purposes without prior authorization from the depositor.
Vector constructed from pOSEX3 (ATCC 87212)
by replacement of the multiple cloning site with a ribosome binding site, intitiation codon and multiple cloning site from pTrc99A.
One of three vectors (ATCC 87214-87216) differing only in the reading frame of the multiple cloning site.
Herbst B, et al. pOSEX: vectors for osmotically controlled and finely tuned gene expression in Escherichia coli. Gene 151: 137-142, 1994. PubMed: 7828862
Erhard Bremer, personal communication
Freeze dried E. coli containing the plasmid.