pOSEX5A (ATCC® 87214)

Applications: expression vectorvector permitting construction of fusion proteins  /  Depositors: E Bremer

Permits and Restrictions

View Permits

Designations pOSEX5A
Depositors E Bremer
Biosafety Level 1
Vector Information
Size (kb): 4.4429998397827150
Vector: pOSEX5A (plasmid)
Promoters: Promoter for expression proU
Construction: pOSEX3
Marker(s):ampR
Construct size (kb): 4.442999839782715
Features: initiation codon: ATG
marker(s): ampR
promoter for expression: proU
replicon: pMB1
replicon: rop (copy number control)
MCS: NcoI...HindIII
transcription terminator: rrnB T1
transcription terminator: rrnB T2
coding sequence: proV 5' sequence
Applications
expression vector
vector permitting construction of fusion proteins
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.4; HindIII--4.4; PvuII--4.4.
Expression can be induced in cells grown in low osmolarity media by the addition of sodium chloride.
Expression vector allowing osmotically controlled expression of cloned inserts directed by the E. coli proU promoter.
Absence of a stop codon between the proV' sequence and the ATG start codon allows production of a fusion protein between ProV and the cloned insert. Insertion of a stop codon at this junction may reduce the yield of recombinant protein.
This material is being provided with the explicit understanding that it not be used for commercial purposes without prior authorization from the depositor.
Vector constructed from pOSEX3 (ATCC 87212) by replacement of the multiple cloning site with a ribosome binding site, intitiation codon and multiple cloning site from pTrc99A.
One of three vectors (ATCC 87214-87216) differing only in the reading frame of the multiple cloning site.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Herbst B, et al. pOSEX: vectors for osmotically controlled and finely tuned gene expression in Escherichia coli. Gene 151: 137-142, 1994. PubMed: 7828862

Erhard Bremer, personal communication

Shipped freeze-dried
Shipping Information

 Freeze dried E. coli containing the plasmid.

Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map