Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Size (kb): 4.4320001602172850
Vector: pOSEX3 (plasmid)
Promoters: Promoter for expression proU
Construct size (kb): 4.432000160217285
Features: marker(s): ampR
promoter for expression: proU
replicon: rop (copy number control)
transcription terminator: rrnB T1
transcription terminator: rrnB T2
coding sequence: proV 5' sequence
Restriction digests of the clone give the following sizes (kb): BamHI--4.4; HindIII--4.4; PvuII--4.4.
Expression can be induced in cells grown in low osmolarity media by the addition of sodium chloride.
Vector does not provide a ribosome binding site or initiation codon.
Expression vector allowing osmotically controlled expression of cloned inserts directed by the E. coli proU promoter.
This material is being provided with the explicit understanding that it not be used for commercial purposes without prior authorization from the depositor.
Vector constructed from pOSEX2 (ATCC 87211)
by deletion of a portion of the pBR322 backbone.
Herbst B, et al. pOSEX: vectors for osmotically controlled and finely tuned gene expression in Escherichia coli. Gene 151: 137-142, 1994. PubMed: 7828862
Erhard Bremer, personal communication