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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 4.4320001602172850 Vector: pOSEX3 (plasmid) Promoters: Promoter for expression proU Construction: pOSEX2 Marker(s):ampR Construct size (kb): 4.432000160217285 Features: marker(s): ampR
promoter for expression: proU
replicon: pMB1
replicon: rop (copy number control)
MCS: BamHI...HindIII
transcription terminator: rrnB T1
transcription terminator: rrnB T2
coding sequence: proV 5' sequence
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Comments
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Restriction digests of the clone give the following sizes (kb): BamHI--4.4; HindIII--4.4; PvuII--4.4. Expression can be induced in cells grown in low osmolarity media by the addition of sodium chloride. Vector does not provide a ribosome binding site or initiation codon. Expression vector allowing osmotically controlled expression of cloned inserts directed by the E. coli proU promoter. This material is being provided with the explicit understanding that it not be used for commercial purposes without prior authorization from the depositor. Vector constructed from pOSEX2 ( ATCC 87211) by deletion of a portion of the pBR322 backbone. |
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References
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Herbst B, et al. pOSEX: vectors for osmotically controlled and finely tuned gene expression in Escherichia coli. Gene 151: 137-142, 1994. PubMed: 7828862
Erhard Bremer, personal communication
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