pUK (ATCC^® 87209^™)

Applications: expression vector  /  Depositors: B Huang

Designations	pUK
Depositors	B Huang
Biosafety Level	1
Vector Information	Size (kb): 2.4300000667572020 Vector: pUK (plasmid) Promoters: Promoter tac Construction: pUC19, pKK223-3 Marker(s):ampR Construct size (kb): 2.430000066757202 Features: marker(s): ampR promoter: tac replicon: pMB1 MCS: HindIII-EcoRI ribosome-binding site: Shine-Dalgarno transcription terminator: rrnB
Applications	expression vector
Comments	Restriction digests of the clone give the following sizes (kb): EcoRI--2.4; BamHI--2.4; HindIII--2.4. A small, high-copy-number vector suitable for both in vitro (using the coupled transcription/translation system of Zubay) and in vivo expression.
Media	ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions	Temperature: 37.0°C
References	Huang B, et al. A small, high-copy-number vector suitable for both in vitro and in vivo gene expression. Gene 151: 143-145, 1994. PubMed: 7828863
Shipped	FD

Product Sheet	Product Sheet
Attachments	Vector Map