pLDR31 [WM2191] (ATCC® 87203)

Applications: contains easily purifiable cassette(s) for construction cI857 - PR - copB - copA - repA cassette  /  Depositors: W Messer

Permits and Restrictions

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Designations pLDR31 [WM2191]
Depositors W Messer
Biosafety Level 1
Vector Information
Size (kb): 6.0000000000000000
Vector: pLDR31 (plasmid)
Construction: pOU71
Marker(s):ampR
Construct size (kb): 6.0
Features: other: cI857 - PR - copB - copA - repA cassette
MCS: ClaI...EcoRI
coding sequence: bla
Applications
contains easily purifiable cassette(s) for construction cI857 - PR - copB - copA - repA cassette
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.6; EcoRI--6.6; NotI--3.5, 2.3, 1.3, 0.8.
Plasmid with a NotI cassette (3.451 kb) containing an R1 replication origin.
The vector component of this construct contains unknown sequence. This should be used only to excise the origin-containing cassette.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348

Walter Messer, personal communication

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map