2P2 (ATCC® 87189™)

Organism: Homo sapiens, human  /  Tissue: Library  /  Depositors: F Kao

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Designations 2P2
Species Homo sapiens, human
Depositors F Kao
Vector
Chromosome: 2p23-p21
DNA: genomic
Digest: Complete
Insert size range: 50 - 600 bp
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Comments
Approximately 45% of the clones contain repetitive sequences. Of 94 single copy clones tested, 60 (64%) hybridized to chromosome 2.
Constructed by microdissecting fragments from 20 chromosomes, treating with proteinase K, extracting with phenol, ligating to an MboI linker-adaptor, amplifying by PCR, digesting with MboI, and ligating to pUC19.
References

Kao FT, et al. A region-specific microdissection library for human chromosome 2p23-->p21 and the analysis of an interstitial deletion of 2p21. Cytogenet. Cell Genet. 68: 17-18, 1995. PubMed: 7956351

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Product Sheet
Product Sheet
Product Sheet