2P1 (ATCC® 87188)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: F Kao

Permits and Restrictions

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Designations 2P1
Species Homo sapiens, human
Depositors F Kao
Vector
Chromosome: 2p23-p25
DNA: genomic
Digest: Complete
Insert size range: 50-600 bp
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Comments
Approximately 40% of the clones contain repetitive sequences. Of 48 single copy clones tested, 26 (54%) hybridized to chromosome 2.
Constructed by microdissecting fragments from 20 chromosomes, treating with proteinase K, extracting with phenol, ligating to an MboI linker-adaptor, amplifying by PCR, digesting with MboI, and ligating to pUC19.
References

Yu J, et al. A region-specific microdissection library for human chromosome 2p23-p25 and the analysis of an interstitial deletion of p23.3-p25.1. Hum. Genet. 93: 557-562, 1994. PubMed: 8168834

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