These permits may be required for shipping this product:
Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
K Ippen-Ihler, S Maneewannakul
Size (kb): 5.0409998893737790
Vector: pKSM715 (phagemid)
Promoters: Promoter for in vitro transcription T7lac
Construction: pKSM713, pETIIa
Construct size (kb): 5.040999889373779
Features: insert detection: lacZalpha
promoter for expression: lacUV5
promoter for in vitro transcription: T7lac
repressor gene: lacI
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BamHI--5.0; EcoRI--5.0; PstI--4.3, 0.6.
Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglII--3.47; EcoRI--0.39; KpnI--0.36, 5.02; NcoI--0.33, 2.87; PstI--2.58, 3.25; PvuI--0.51; PvuII--0.48, 3.20, 3.56, 3.90, 3.99; SacI--0.38, 5.03; SalI--2.57.
Differs from pKSM713 (ATCC 87159)
only in the substitution of lacI for lacIq.
Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823