|
Permits
|
These permits may be required for shipping this product:
-
Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
|
|
Depositors
|
K Ippen-Ihler, S Maneewannakul
|
|
Vector Information
|
Size (kb): 5.0409998893737790 Vector: pKSM715 (phagemid) Promoters: Promoter for in vitro transcription T7lac Construction: pKSM713, pETIIa Marker(s):kanR Construct size (kb): 5.040999889373779 Features: insert detection: lacZalpha
marker(s): kanR
promoter for expression: lacUV5
promoter for in vitro transcription: T7lac
replicon: fd
replicon: pMB1
repressor gene: lacI
MCS: BamHI...EcoRI
terminator: trpA
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB
|
|
Applications
|
expression vector vector permitting RNA synthesis in vitro vector permitting production of single-stranded DNA vector permitting visual detection of recombinants
|
|
Comments
|
Restriction digests of the clone give the following sizes (kb): BamHI--5.0; EcoRI--5.0; PstI--4.3, 0.6. Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglII--3.47; EcoRI--0.39; KpnI--0.36, 5.02; NcoI--0.33, 2.87; PstI--2.58, 3.25; PvuI--0.51; PvuII--0.48, 3.20, 3.56, 3.90, 3.99; SacI--0.38, 5.03; SalI--2.57. Differs from pKSM713 ( ATCC 87159) only in the substitution of lacI for lacIq. Expression vector.
|
|
References
|
Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823
|