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K Ippen-Ihler, S Maneewannakul
Size (kb): 3.7409999370574950
Vector: pKSM712 (phagemid)
Promoters: Promoter for in vitro transcription T7lac
Construction: pKSM710, pUC4-KIXX
Construct size (kb): 3.740999937057495
Features: insert detection: lacZalpha
promoter for expression: lacUV5
promoter for in vitro transcription: T7lac
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BamHI--3.75; EcoRI--3.75; PstI--3.75.
Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglII--2.89; EcoRI--0.39; NcoI--0.33, 3.49; PstI--3.11; PvuI--0.51; PvuII--0.48, 2.80, 3.16.
Differs from pKSM711 (ATCC 87157)
only in the orientation of the kanamycin resistance gene.
Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823