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Depositors
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K Ippen-Ihler, S Maneewannakul
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Vector Information
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Size (kb): 3.7409999370574950 Vector: pKSM711 (phagemid) Promoters: Promoter for in vitro transcription T7lac Construction: pKSM710, pUC4-KIXX Marker(s):kanR Construct size (kb): 3.740999937057495 Features: insert detection: lacZalpha
marker(s): kanR
promoter for expression: lacUV5
promoter for in vitro transcription: T7lac
replicon: fd
replicon: pMB1
MCS: BamHI...EcoRI
transcription terminator: T7
transcription terminator: fd
transcription terminator: rrnB
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Applications
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expression vector vector permitting RNA synthesis in vitro vector permitting production of single-stranded DNA vector permitting visual detection of recombinants
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Comments
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Restriction digests of the clone give the following sizes (kb): BamHI--3.7; EcoRI--3.7; PstI--3.7. Vector contains the following restriction sites (approximate kb from nt 1): BamHI--0.34; BglII--3.42; EcoRI--0.39; NcoI--0.33, 2.82; PstI--3.20; PvuI--0.51; PvuII--0.48, 3.15, 3.51. Differs from pKSM712 ( ATCC 87158) only in the orientation of the kanamycin resistance gene. Expression vector.
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References
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Maneewannakul S, et al. The pKSM710 vector cassette provides tightly regulated lac and T7lac promoters and strategies for manipulating N-terminal protein sequences. Plasmid 31: 300-307, 1994. PubMed: 8058823
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