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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 5.2750000953674320 Vector: pBSL193 (phagemid) Promoters: Promoter for in vitro transcription T7 (phi10) Construction: pBluescript Marker(s):ampR,tetR Construct size (kb): 5.275000095367432 Features: marker(s): ampR
promoter for in vitro transcription: T7 (phi10)
replicon: f1
replicon: pMB1
MCS: KpnI...SacI MluI
MCS: MluI KpnI...SacI
coding sequence: tetR
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Applications
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contains easily purifiable cassette(s) for construction omega-tet
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Comments
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Restriction digests of the clone give the following sizes (kb):BamHI--3.0, 2.3; EcoRI--3.0, 1.45, 0.6, 0.4; KpnI--3.0, 2.3; MluI--3.15, 2.2; SstI--3.0, 2.35. Plasmid useful for generation of marked deletions and insertions in cloned DNA in vitro. Contains an omega - Tc element flanked by multiple cloning sites duplicated in tandem orientation. Corresponds to the EcoRI/StyI fragment of pBR322, with BamHI, SalI, AccI, HincII, and HindIII sites removed.
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References
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Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718
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