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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 4.2519998550415040 Vector: pBSL175 (plasmid) Promoters: Promoter for in vitro transcription T7 (phi10) Construction: pSU18 Marker(s):cmlR,spcR,strR Construct size (kb): 4.251999855041504 Features: marker(s): cmlR
promoter for in vitro transcription: T7 (phi10)
replicon: p15A
MCS: KpnI...SacI MluI
MCS: MluI KpnI...SacI
coding sequence: aadA (spcR, strR)
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Applications
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contains easily purifiable cassette(s) for construction omega-aadA1
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Comments
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Restriction digests of the clone give the following sizes (kb): MluI--2.2, 2.1; EcoRI--2.2, 2.1; BamHI--2.2, 2.1. Plasmid useful for vector construction. Contains an omega-Gm element (aadA, strR, spcR) flanked by multiple cloning sites duplicated in tandem orientation.
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References
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Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718
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