pBSL175 (ATCC® 87150)

Applications: contains easily purifiable cassette(s) for construction omega-aadA1  /  Depositors: MF Alexeyev

Permits and Restrictions

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Designations pBSL175
Depositors MF Alexeyev
Biosafety Level 1
Vector Information
Size (kb): 4.2519998550415040
Vector: pBSL175 (plasmid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construction: pSU18
Marker(s):cmlR,spcR,strR
Construct size (kb): 4.251999855041504
Features: marker(s): cmlR
promoter for in vitro transcription: T7 (phi10)
replicon: p15A
MCS: KpnI...SacI MluI
MCS: MluI KpnI...SacI
coding sequence: aadA (spcR, strR)
Applications
contains easily purifiable cassette(s) for construction omega-aadA1
Comments
Restriction digests of the clone give the following sizes (kb): MluI--2.2, 2.1; EcoRI--2.2, 2.1; BamHI--2.2, 2.1.
Plasmid useful for vector construction. Contains an omega-Gm element (aadA, strR, spcR) flanked by multiple cloning sites duplicated in tandem orientation.
Media ATCC® Medium 1632: LB medium (ATCC medium 1065) with 10 mcg/ml chloramphenicol
Growth Conditions
Temperature: 37.0°C
References

Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718

Shipped frozen
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