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pBSL168 (ATCC^® 87149^™)

Applications: contains easily purifiable cassette(s) for construction wild type beta-lactamase / Depositors: MF Alexeyev

pBSL168 ATCC^® 87149^™

freeze-dried

For-Profit: $190.00 Non-Profit: $190.00

Qty:

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Designations	pBSL168
Depositors	MF Alexeyev
Biosafety Level	1
Vector Information	Size (kb): 3.3570001125335690 DESCRIPTION OF VECTOR COMPONENT: Name of vector: sBSL150 Intact vector size: 2.200 Type of vector: plasmid Vector end: mLuI Vector end: mLuI Cloning sites: Polylinker sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI NotI/EagI BstXI SacII SacI mLuI Construction: pSU18 Host range: Escherichia coli Features (with orientation and position when available): MCS: SacI...KpnI mLuI, -> MCS: mLuI KpnI...SacI, -> replicon: p15A marker(s): cmLR, <- Cross references: Vector: pBSL168 (plasmid) Promoters: Promoter for in vitro transcription T7 (phi10) Construction: pSU18 Marker(s):ampR,cmlR Construct size (kb): 3.357000112533569 Features: marker(s): cmlR promoter for in vitro transcription: T7 (phi10) replicon: p15A MCS: KpnI...SacI MluI MCS: SacI...KpnI MluI coding sequence: bla (ampR)
Applications	contains easily purifiable cassette(s) for construction wild type beta-lactamase
Comments	Restriction digests of the clone give the following sizes (kb): BamHI--2.0, 1.35; EcoRI--2.05, 1.25; MluI--2.1, 1.15; SstI--1.9, 1.4; NcoI/BamHI--1.4, 1.1, 0.9; NotI/ScaI--1.1, 0.9, 0.8, 0.7. Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA in vitro. Contains a beta-lactamase (ampR) cassette flanked by multiple cloning sites duplicated in inverted orientation. The PstI and HincII sites in the bla gene have been removed.
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions	Temperature: 37.0°C
References	Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718
Shipped	FD
Shipping Information	Distributed: freeze-dried

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Product Sheet	Product Sheet
Attachments	Vector Map