pBSL142 (ATCC® 87147)

Applications: contains easily purifiable cassette(s) for construction omega - aacC1  /  Depositors: MF Alexeyev

Designations pBSL142
Depositors MF Alexeyev
Biosafety Level 1
Host
Distribution host: Escherichia coli DH5
Vector Information
Size (kb): 4.6690001487731930
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: sBSL98
Intact vector size: 3.100
Type of vector: phagemid
Vector end: mLuI
Vector end: mLuI
Cloning sites:
Polylinker sites: KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI
SpeI XbaI NotI/EagI BstXI SacII SacI mLuI
Construction: pBluescript
Host range: Escherichia coli
Features (with orientation and position when available):
MCS: KpnI...SacI mLuI, ->
MCS: mLuI KpnI...SacI, ->
replicon: pMB1
marker(s): ampR, <-
replicon: f1, <-
Cross references:
Vector: pBSL142 (phagemid)
Promoters: Promoter for in vitro transcription f1T7 (phi10)
Construction: pBluescript
Marker(s):ampR,gtmR
Construct size (kb): 4.669000148773193
Features: marker(s): ampR
promoter for in vitro transcription: f1T7 (phi10)
replicon: f1
replicon: pMB1
MCS: KpnI...SacI MluI
MCS: MluI KpnI...SacI
coding sequence: aacC1 (gtmR)
Applications
contains easily purifiable cassette(s) for construction omega - aacC1
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--2.8, 1.6; EcoRI--2.95, 1.75; KpnI--2.8, 1.65; MluI--2.9, 1.6; SstI--2.8, 1.7.
Plasmid useful for generation of marked deletions and insertions in cloned DNA in vitro. Contains an omega - Gm element (aacC1, gtmR) flanked by multiple cloning sites duplicated in tandem orientation.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map