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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 4.8420000076293950 Vector: pBSL119 (phagemid) Promoters: Promoter for in vitro transcription T7 (phi10) Construction: pBluescript Marker(s):ampR,cmlR Construct size (kb): 4.842000007629395 Features: marker(s): ampR
other: omega element
promoter for in vitro transcription: T7 (phi10)
replicon: f1
replicon: pMB1
MCS: KpnI...SacI MluI
MCS: MluI KpnI...SacI
coding sequence: cmlR (cat)
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Applications
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contains easily purifiable cassette(s) for construction omega-cat
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Comments
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Restriction digests of the clone give the following sizes (kb): MluI--3.1, 1.8; EcoRI--2.9, 1.9; BamHI--2.9, 1.8. Contains an omega-Cm element acetyltransferase (cmlR) and multiple cloning sites duplicated in tandem orientation. Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA. The EcoRI site was removed.
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References
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Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718
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