pBSL119 (ATCC® 87142)

Applications: contains easily purifiable cassette(s) for construction omega-cat  /  Depositors: MF Alexeyev

Permits and Restrictions

View Permits

Designations pBSL119
Depositors MF Alexeyev
Biosafety Level 1
Vector Information
Size (kb): 4.8420000076293950
Vector: pBSL119 (phagemid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construction: pBluescript
Marker(s):ampR,cmlR
Construct size (kb): 4.842000007629395
Features: marker(s): ampR
other: omega element
promoter for in vitro transcription: T7 (phi10)
replicon: f1
replicon: pMB1
MCS: KpnI...SacI MluI
MCS: MluI KpnI...SacI
coding sequence: cmlR (cat)
Applications
contains easily purifiable cassette(s) for construction omega-cat
Comments
Restriction digests of the clone give the following sizes (kb): MluI--3.1, 1.8; EcoRI--2.9, 1.9; BamHI--2.9, 1.8.
Contains an omega-Cm element acetyltransferase (cmlR) and multiple cloning sites duplicated in tandem orientation.
Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA.
The EcoRI site was removed.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map