pBSL99 (ATCC® 87141)

Applications: contains easily purifiable cassette(s) for construction aminoglycoside phosphotransferase kanamycin resistance, neomycin resistance  /  Depositors: MF Alexeyev

Permits and Restrictions

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Designations pBSL99
Depositors MF Alexeyev
Biosafety Level 1
Vector Information
Size (kb): 4.2859997749328610
Vector: pBSL99 (phagemid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construction: pBluescript
Marker(s):ampR,kanR
Construct size (kb): 4.285999774932861
Features: marker(s): ampR
promoter for in vitro transcription: T7 (phi10)
replicon: f1
replicon: pMB1
MCS: MluI KpnI...SacI
MCS: SacI...KpnI MluI
coding sequence: nptII (kanR)
Applications
contains easily purifiable cassette(s) for construction aminoglycoside phosphotransferase kanamycin resistance, neomycin resistance
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.75, 1.4; KpnI--2.9, 1.25; MluI--2.9, 1.25; SstI--2.6, 1.5.
Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA.
SphI and PstI sites in the nptII gene were removed by site-directed mutagenesis.
Contains a nptII (kanR) cassette flanked by multiple cloning sites duplicated in inverted orientation.
pBSL97 (ATCC 87140) and pBSL99 (ATCC 87141) differ in the orientations of the multiple cloning sites.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718

Shipped freeze-dried
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