pBSL99 (ATCC® 87141)

Applications: contains easily purifiable cassette(s) for construction aminoglycoside phosphotransferase kanamycin resistance, neomycin resistance  /  Depositors: MF Alexeyev

Designations pBSL99
Depositors MF Alexeyev
Biosafety Level 1
Vector Information
Size (kb): 4.2859997749328610
Vector: pBSL99 (phagemid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construction: pBluescript
Marker(s):ampR,kanR
Construct size (kb): 4.285999774932861
Features: marker(s): ampR
promoter for in vitro transcription: T7 (phi10)
replicon: f1
replicon: pMB1
MCS: MluI KpnI...SacI
MCS: SacI...KpnI MluI
coding sequence: nptII (kanR)
Applications
contains easily purifiable cassette(s) for construction aminoglycoside phosphotransferase kanamycin resistance, neomycin resistance
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.75, 1.4; KpnI--2.9, 1.25; MluI--2.9, 1.25; SstI--2.6, 1.5.
Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA.
SphI and PstI sites in the nptII gene were removed by site-directed mutagenesis.
Contains a nptII (kanR) cassette flanked by multiple cloning sites duplicated in inverted orientation.
pBSL97 (ATCC 87140) and pBSL99 (ATCC 87141) differ in the orientations of the multiple cloning sites.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map