pBSL15 (ATCC® 87128)

Applications: contains easily purifiable cassette(s) for construction aminoglycoside phosphotransferase kanamycin resistance, neomycin resistancevector permitting positive selection for inserts  /  Depositors: MF Alexeyev

Permits and Restrictions

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Designations pBSL15
Depositors MF Alexeyev
Biosafety Level 1
Vector Information
Size (kb): 3.9000000953674320
Vector: pBSL15 (plasmid)
Construction: pUC18, pK18
Marker(s):ampR,kanR
Construct size (kb): 3.900000095367432
Features: marker(s): ampR
marker(s): kanR
replicon: pMB1
MCS: HindIII...EcoRI
MCS: NheI...HindIII
Applications
contains easily purifiable cassette(s) for construction aminoglycoside phosphotransferase kanamycin resistance, neomycin resistance
vector permitting positive selection for inserts
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.8, 1.2; BamHI--2.75, 1.3; HindIII--2.7, 1.4.
The nptII gene was modified by site-directed mutagenesis to remove PstI and SphI sites.
An inverted repeat of the 271 bp NdeI/EcoRI fragment of pUC18 surrounds the kanR cassette. If this vector is ligated without a stuffer fragment, this inverted repeat prevents replication, thus providing positive selection for plasmids with inserts.
Plasmid with an easily purified kanR cassette (flanked by inverted repeats of the pUC18 multiple cloning sequence) for construction. pBSL15 and pBSL86 differ in the orientation of the repeats.
Media LB plus ampicillin (50 mcg/ml) plus kanamycin (50 mcg/ml)
Growth Conditions
Temperature: 37.0°C
References

Alexeyev MF. Three kanamycin resistance gene cassettes with different polylinkers. BioTechniques 18: 52-55, 1995. PubMed: 7702853

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet
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