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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Host
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Distribution host: Escherichia coli HB101 ( ATCC 33694) |
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Vector Information
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Size (kb): 3.9000000953674320 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: cosmid
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752 Vector: pBSL14 (plasmid) Construction: pUC18, pK18 Marker(s):ampR,kanR Construct size (kb): 3.900000095367432 Features: marker(s): ampR
marker(s): kanR
replicon: pMB1
MCS: HindIII...EcoRI
MCS: HindIII...NheI
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Applications
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contains easily purifiable cassette(s) for construction aminoglycoside phosphotransferase kanamycin resistance, neomycin resistance
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Comments
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Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.8, 1.1; HindIII--2.8, 1.1; BamHI--2.7, 1.2. The nptII gene was modified by site-directed mutagenesis to remove PstI and SphI sites. Plasmid with an easily purified kanR cassette (flanked by direct repeats of the pUC18 multiple cloning site) for use in construction.
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References
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Alexeyev MF. Three kanamycin resistance gene cassettes with different polylinkers. BioTechniques 18: 52-55, 1995. PubMed: 7702853
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Shipping Information
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Distributed: freeze-dried
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