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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 2.8959999084472660 Vector: pTZ19RBS.K (phagemid) Promoters: Promoter T7 (phi10) Construction: pTZ19R Marker(s):ampR Construct size (kb): 2.895999908447266 Features: insert detection: lacZ'
marker(s): ampR
promoter: T7 (phi10)
promoter: lacOP
replicon: f1
replicon: pMB1
MCS: EcoRI...HindIII
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Applications
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vector permitting construction of fusion proteins vector permitting positive selection for inserts vector permitting visual detection of recombinants
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Comments
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Restriction digests of the clone give the following sizes (kb): BamHI--2.9, EcoRI--2.9, PstI--2.9. Cloning into the BamHI site followed by transformation into an EcoK r+ m+ host, increases the relative transformation efficiency of recombinant molecules, provided that the cloned insert is free of non-methylated EcoK sites. Vector allowing positive selection and blue/white color detection for cloned inserts. Vector constructed from pTZ19R by elimination of the EcoK restriction site in the ampR region and creation of a new EcoK site within the polylinker, containing a BamHI site as the central hexamer of the EcoK recognition sequence.
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References
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De Backer O, et al. Positive selection of recombinant plasmids based on the EcoK restriction activity of Escherichia coli K-12. Gene 150: 197-198, 1994. PubMed: 7959053
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