pTZ19RBS.K (ATCC® 87124)

Applications: vector permitting construction of fusion proteinsvector permitting positive selection for insertsvector permitting visual detection of recombinants  /  Depositors: O De Backer

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Designations pTZ19RBS.K
Depositors O De Backer
Biosafety Level 1
Vector Information
Size (kb): 2.8959999084472660
Vector: pTZ19RBS.K (phagemid)
Promoters: Promoter T7 (phi10)
Construction: pTZ19R
Marker(s):ampR
Construct size (kb): 2.895999908447266
Features: insert detection: lacZ'
marker(s): ampR
promoter: T7 (phi10)
promoter: lacOP
replicon: f1
replicon: pMB1
MCS: EcoRI...HindIII
Applications
vector permitting construction of fusion proteins
vector permitting positive selection for inserts
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--2.9, EcoRI--2.9, PstI--2.9.
Cloning into the BamHI site followed by transformation into an EcoK r+ m+ host, increases the relative transformation efficiency of recombinant molecules, provided that the cloned insert is free of non-methylated EcoK sites.
Vector allowing positive selection and blue/white color detection for cloned inserts.
Vector constructed from pTZ19R by elimination of the EcoK restriction site in the ampR region and creation of a new EcoK site within the polylinker, containing a BamHI site as the central hexamer of the EcoK recognition sequence.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

De Backer O, et al. Positive selection of recombinant plasmids based on the EcoK restriction activity of Escherichia coli K-12. Gene 150: 197-198, 1994. PubMed: 7959053

Shipped freeze-dried
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