Size (kb): 2.8959999084472660
Vector: pTZ19RBS.K (phagemid)
Promoters: Promoter T7 (phi10)
Construct size (kb): 2.895999908447266
Features: insert detection: lacZ'
promoter: T7 (phi10)
vector permitting construction of fusion proteins
vector permitting positive selection for inserts
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): BamHI--2.9, EcoRI--2.9, PstI--2.9.
Cloning into the BamHI site followed by transformation into an EcoK r+ m+ host, increases the relative transformation efficiency of recombinant molecules, provided that the cloned insert is free of non-methylated EcoK sites.
Vector allowing positive selection and blue/white color detection for cloned inserts.
Vector constructed from pTZ19R by elimination of the EcoK restriction site in the ampR region and creation of a new EcoK site within the polylinker, containing a BamHI site as the central hexamer of the EcoK recognition sequence.
De Backer O, et al. Positive selection of recombinant plasmids based on the EcoK restriction activity of Escherichia coli K-12. Gene 150: 197-198, 1994. PubMed: 7959053