pMR101
87115 ™
- Clone type
- Vector
- Applications
-
Molecular biology
- Product format
- Freeze-dried
Documentation
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
Detailed product information
General
- Specific applications
-
expression vectorvector permitting production of single-stranded DNA
Characteristics
- Comments
-
Restriction digests of the clone give the following sizes (kb): BamHI--4.1; BstEII/PstI--3.0, 1.08; SalI--4.1.There is an error in the reference in figures 1 and 2. The restriction sites, XbaI and BglII, are drawn in reverse order on the maps for pMR101.Expression vector (T7-based) with a kanR marker and a P15A replicon compatible with ColE1-derived plasmids. Particularly useful for co-transformation with ColE1-based ampR T7 expression vectors and the production of two proteins in the same cell.If used in an Escherichia coli strain that expresses T7 polymerase under the control of the lacUV5 promotor (such as BL21(DE3)), addition of IPTG can result in high levels of recombinant protein production.Use of 5'NcoI and 3'BamHI cloning sites is similar to that of other expression systems, which facilitiates transfer of genes into these pMR vectors.
- Mycoplasma contamination
- Not detected
Vector information
- Construct size (kb)
- 4.099999904632568
- Intact vector size
- 4.100
- Vector name
- pMR101 (phagemid)
- Type of vector
- phagemid
- Construction
- pTM201/NS3-3, pET8c, pACYC177, pET11d
- Host range
-
Escherichia coli
- Vector information
- Other unique sites: EcoNI, SalI, BstEII, PstI, BglII, XbaI
- Cloning sites
-
NcoI; BamHI
- Markers
-
kanR
- Operator
-
lac, <-, 3788-3804
- Promoters
- T7 (phi10), <-, 3808-3827
- Replicon
- M13, →, 11-467; p15A, →, 1585-1587
Handling information
- Host
-
Escherichia coli JM101 (ATCC 33876)
- Medium
- Temperature
-
37°C
- Handling notes
-
Restriction digests of the clone give the following sizes (kb): BamHI--4.1;
BstEII/PstI--3.0, 1.08; SalI--4.1.
- ATCC staff
There is an error in the reference in figures 1 and 2. The restriction sites,
XbaI and BglII, are drawn in reverse order on the maps for pMR101.
- personal communication
Expression vector (T7-based) with a kanR marker and a P15A replicon compatible
with ColE1-derived plasmids. Particularly useful for co-transformation with
ColE1-based ampR T7 expression vectors and the production of two proteins in the
same cell.
- Gene 144: 59-62, 1994
Use of 5'NcoI and 3'BamHI cloning sites is similar to that of other expression
systems, which facilitiates transfer of genes into these pMR vectors.
- Gene 144: 59-62, 1994
If used in an Escherichia coli strain that expresses T7 polymerase under the
control of the lacUV5 promotor (such as BL21(DE3)), addition of IPTG can result
in high levels of recombinant protein production.
- Gene 144: 59-62, 1994
History
- Depositors
- L Regan, M Munson
- Special collection
-
NCRR Contract
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
-
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
-
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Permits & Restrictions
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Images
Frequently Asked Questions
References
Curated Citations
Munson M, et al. ColE1-compatible vectors for high-level expression of cloned DNAs from the T7 promoter. Gene 144: 59-62, 1994. PubMed: 8026759
Mary Munson, personal communication
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