pJC40 (ATCC® 87114)

Applications: encodes removable tag for protein isolationexpression vectorvector for generating nested deletions of cloned inserts  /  Depositors: J Clos

Designations pJC40
Depositors J Clos
Biosafety Level 1
Vector Information
Size (kb): 2.4000000953674320
Vector: pJC40 (plasmid)
Promoters: Promoter for expression T7 (phi10)
Construction: pJC20, pET16b
Marker(s):ampR
Construct size (kb): 2.400000095367432
Features: marker(s): ampR
other: Factor Xa cleavage site
other: poly (His) 10
promoter for expression: T7 (phi10)
replicon: pMB1
MCS: NdeI...ApaI
ribosome-binding site: Shine-Dalgarno
terminator: T phi
Applications
encodes removable tag for protein isolation
expression vector
vector for generating nested deletions of cloned inserts
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.4; XbaI--2.4; HindIII/PvuI-- 1.5, 0.8.
High-copy-number expression vector (T7-based) that can be used for preparation of nested deletions.
Also encodes an amino-terminal histidine sequence of 10 residues and a factor Xa cleavage site allowing purification of a recombinant gene product by metal chelate chromatography and removal of the amino terminus.
Growth Conditions
Temperature: 37.0°C
References

Clos J, Brandau S. pJC20 and pJC40--two high-copy-number vectors for T7 RNA polymerase-dependent expression of recombinant genes in Escherichia coli. Protein Expr. Purif. 5: 133-137, 1994. PubMed: 8054844

Clos J, et al. Molecular cloning and expression of hexameric Drosophila heat shock factor subject to negative regulation. Cell 63: 1085-1097, 1990. PubMed: 2257625

Shipped freeze-dried
Shipping Information

 Freeze dried E. coli containing the plasmid

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