2P4 (ATCC® 87104)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: F Kao

Permits and Restrictions

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Designations 2P4
Species Homo sapiens, human
Depositors F Kao
Vector
Chromosome: 2p11-p13
DNA: genomic
Digest: Complete
Insert size range: 100-800 bp
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Purity: 90%. Approximately 30-40% of clones contain non-repetitive sequences.
Constructed by microdissecting fragments from 20 chromosomes, treating with proteinase K, extracting with phenol, ligating to an MboI linker-adaptor, and amplifying by PCR.
References

Yu J, et al. Region-specific microdissection library and single-copy microclones for human chromosome 2p11-p13. Somatic Cell Mol. Genet. 20: 133-136, 1994. PubMed: 8009366

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