pT7/Nde (ATCC® 87050)

Other IDs:

Nucleotide (GenBank) : U25273 Expression cloning vector pT7/Nco, complete sequence.

 /  Applications: expression vector  /  Depositors: RS Haun

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Designations pT7/Nde
Depositors RS Haun
Other IDs

Nucleotide (GenBank) : U25273 Expression cloning vector pT7/Nco, complete sequence.

Biosafety Level 1
Vector Information
Size (kb): 2.9000000953674320
Vector: pT7/Nde (plasmid)
Promoters: Promoter SP6
Construction: pGEM-3, pET-3a
Marker(s):ampR
Construct size (kb): 2.900000095367432
Features: marker(s): ampR
promoter: SP6
promoter: T7 (phi10)
replicon: pMB1
MCS: HindIII...XbaI
ribosome-binding site: gene 10
Applications
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): XbaI--2.90; HindIII--2.90; NdeI--2.90.
The NdeI site (CATATG) includes the initiator methionine codon. The NdeI cloning site can also be used in conjunction with the downstream BamHI site.
Differs from pT7/NcoI (ATCC 87051) only in the cloning site and reading frame for the insert.
Expression vector, maintained at high copy number.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Hong JX, et al. Effect of ADP-ribosylation factor amino-terminal deletions on its GTP-dependent stimulation of cholera toxin activity. J. Biol. Chem. 269: 9743-9745, 1994. PubMed: 8144566

Shipped freeze-dried
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