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pDO192

87043

Clone type
Vector
Applications
Molecular biology
Product format
Freeze-dried
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Price: $620.00 EA
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
vector containing primer sites useful for sequencing
vector for generating nested deletions of cloned inserts
vector permitting visual detection of recombinants

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.8; HindIII--6.8; XbaI--5.8, 0.9.
Plasmid contains the following restriction sites (approximate kb from nt 1): BamHI--3.08; BglI--3.26, 5.34; EcoRI--3.06; KpnI--3.07; HindIII--3.11; PstI--0.65, 5.47; PvuI--3.23, 5.59; SacI--3.07; SmaI--3.08; XbaI--2.11, 3.09.
Resulting fragments are blunted and circularized by ligation, and transformants are selected for either ampicillin or kanamycin resistance.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Cosmid vector containing two compatible origins of replication, for generating bidirectional deletions of large inserts to facilitate sequencing.
A series of overlapping deletion segments can be generated by cutting at the Cos site with lambda terminase, then filling in ends with a alpha phosphorothioate nucleotides, cutting with a restriction enzyme that cuts on one side of the insert,
and then digesting with Exo III for various intervals of time.
AmpR transformants can be sequenced in the clockwise direction using the cosL primer 5'-TCATAAATAGCGAAAACC-3'. KanR transformants can be sequenced in the counterclockwise direction using the cosR primer 5'-ACTTTACGGGTCCTTTCCG-3'.
pDO192 contains 12 unique restriction sites in the polylinker but no restiction sites for any 8 bp cutters.
The order of the major features in the plasmid is: 3' lacZ - HindIII/MCS/EcoRI - 5' lacZ - ori 15 - kanR - cosR/L - ampR - oriP.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
6.735000133514404
Vector name
pDO192 (cosmid)
Construction
pDO184
Vector information
other: lambda cos site
Insert detection
lacZ'
Markers
kanR; ampR
Replicon
oriP; p15A

Handling information

Temperature
30°C

History

Depositors
A Ahmed
Special collection
NCRR Contract
Cross references
GenBank U13761

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Ahmed A. Double-origin vectors for isolating bidirectional deletions useful in DNA sequence analysis. Gene 141: 71-73, 1994. PubMed: 8163177

Ahmed A. Isolation of nested, bidirectional deletions by the use of exonuclease III on double-origin vectors. Gene 151: 97-101, 1994. PubMed: 7828911

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Telephone

US and Puerto Rico
800-638-6597

Outside the US
+1-703-365-2700

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