(ATCC® 87003)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: SJ Elledge

Permits and Restrictions

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Species Homo sapiens, human
Depositors SJ Elledge
Origin
B lymphocyte
Applications
For conversion to plasmid, use Escherichia coli BNN132 (ATCC 47059).
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdaACT
Intact vector size: 42.400
Type of vector: phage
Vector end: XhoI Modification: T-filled
Vector end: XhoI Modification: T-filled
Cloning sites: XhoI
Polylinker sites: BglII EcoRI BamHI XhoI BglII
Construction: lambdaYES, pSE1107
Host range: Escherichia coli
Features (with orientation and position when available):
other: left arm (19.6 kb), ->
restriction site: NotI, ->
other: lox, ->
marker(s): LEU2, <-
replicon: 2 micron
marker(s): ampR, ->
replicon: pMB1
promoter: ADH, ->
other: GAL4 activation domain, ->
MCS: BglII/EcoRI...XhoI/BglII, ->
terminator: ADH, ->
other: lox, ->
restriction site: NotI
other: right arm (15.2 kb, with cI857)
Cross references:
Vector
Tissue: B lymphocyte
DNA: cDNA
Insert size range: to 7.6 kb
Genome Equivalent: 0.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen (volume: 0.2 mL)
Comments
For conversion to plasmid, use Escherichia coli BNN132 (ATCC 47059).
Constructed by ligating cDNAs to adaptors and inserting into T-filled XhoI sites of lambdaACT, as described for lambdaYES.
References

Elledge SJ, et al. lambdaYES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc. Natl. Acad. Sci. USA 88: 1731-1735, 1991. PubMed: 1848010

Durfee T, et al. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555-569, 1993. PubMed: 8384581

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