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(ATCC^® 87002^™)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen / Tissue: Library / Depositors: SJ Elledge

ATCC^® 87002^™

frozen

For-Profit: $400.00 Non-Profit: $400.00

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Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Species	Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors	SJ Elledge
Applications	For conversion to plasmid, use Escherichia coli BNN132 (ATCC 47059).
Vector	DESCRIPTION OF VECTOR COMPONENT: Name of vector: lambdaACT Intact vector size: 42.400 Type of vector: phage Vector end: XhoI Modification: T-filled Vector end: XhoI Modification: T-filled Cloning sites: XhoI Polylinker sites: BglII EcoRI BamHI XhoI BglII Construction: lambdaYES, pSE1107 Host range: Escherichia coli Features (with orientation and position when available): other: left arm (19.6 kb), -> restriction site: NotI, -> other: lox, -> marker(s): LEU2, <- replicon: 2 micron marker(s): ampR, -> replicon: pMB1 promoter: ADH, -> other: GAL4 activation domain, -> MCS: BglII/EcoRI...XhoI/BglII, -> terminator: ADH, -> other: lox, -> restriction site: NotI other: right arm (15.2 kb, with cI857) Cross references:
Vector	DNA: cDNA Insert size range: to 7.6 kb Genome Equivalent: 0.0 Non recombinants (%): 5 Independent recombinants: 100,000,000
Biosafety Level	1
Shipping Information	Distributed: frozen (volume: 0.2 mL)
Comments	For conversion to plasmid, use Escherichia coli BNN132 (ATCC 47059). Constructed by ligating cDNAs to adaptors and inserting into T-filled XhoI sites of lambdaACT, as described for lambdaYES.
Classification	Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References	Elledge SJ, et al. lambdaYES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc. Natl. Acad. Sci. USA 88: 1731-1735, 1991. PubMed: 1848010 Durfee T, et al. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555-569, 1993. PubMed: 8384581

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Product Sheet	Product Sheet