pFN476 (ATCC® 86962)

Applications: expression vector T7 promotervector permitting RNA synthesis in vitrovector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidasevector with low copy number  /  Depositors: P Sankar

Permits and Restrictions

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Designations pFN476
Depositors P Sankar
Biosafety Level 1
Vector Information
Size (kb): 4.320
Vector: pFN476 (plasmid)
Promoters: Promoter lac
Construction: pGEM3Z, pKA101-6
Marker(s):ampR
Construct size (kb): 4.320
Features: insert detection: lacZ'
marker(s): ampR
other: par
promoter: lac
promoter for expression: T7
promoter for in vitro transcription: SP6
replicon: pSC101
replicon: rep (pSC101)
MCS: pGEM-3Z; HindIII...EcoRI
restriction site: BamHI
coding sequence: tnpR
Applications
expression vector T7 promoter
vector permitting RNA synthesis in vitro
vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.2; HindIII--4.2; PstI--3.1, 1.1.
Low copy number vector to express transcripts in vivo from the T7 promoter and to minimize toxic gene effects.
Plasmid contains the pSC101 replicon and is maintained at 1-5 copies per cell.
Low copy number minimizes the effect of uninduced expression of T7 RNA polymerase, reducing transcription of potentially toxic inserts.
T7 RNA polymerase can also be provided by infection of F' hosts with M13mGP1-2 (ATCC 40303).
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Sankar P, et al. Expression analysis of cloned chromosomal segments of Escherichia coli. J. Bacteriol. 175: 5145-5152, 1993. PubMed: 8349554

Shipped frozen