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Permits
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These permits may be required for shipping this product:
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Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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Vector Information
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Size (kb): 4.320 Vector: pFN476 (plasmid) Promoters: Promoter lac Construction: pGEM3Z, pKA101-6 Marker(s):ampR Construct size (kb): 4.320 Features: insert detection: lacZ'
marker(s): ampR
other: par
promoter: lac
promoter for expression: T7
promoter for in vitro transcription: SP6
replicon: pSC101
replicon: rep (pSC101)
MCS: pGEM-3Z; HindIII...EcoRI
restriction site: BamHI
coding sequence: tnpR
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Applications
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expression vector T7 promoter vector permitting RNA synthesis in vitro vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase vector with low copy number
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Comments
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Restriction digests of the clone give the following sizes (kb): EcoRI--4.2; HindIII--4.2; PstI--3.1, 1.1. Low copy number vector to express transcripts in vivo from the T7 promoter and to minimize toxic gene effects. Plasmid contains the pSC101 replicon and is maintained at 1-5 copies per cell. Low copy number minimizes the effect of uninduced expression of T7 RNA polymerase, reducing transcription of potentially toxic inserts. T7 RNA polymerase can also be provided by infection of F' hosts with M13mGP1-2 ( ATCC 40303). |
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References
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Sankar P, et al. Expression analysis of cloned chromosomal segments of Escherichia coli. J. Bacteriol. 175: 5145-5152, 1993. PubMed: 8349554
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