pFL61 Arabidopsis (ATCC® 77500)

Organism: Arabidopsis thaliana Heinhold, mouse-ear cress  /  Clone Type: Library  /  Depositors: F Lacroute

Permits and Restrictions

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Designations pFL61 Arabidopsis
Species Arabidopsis thaliana Heinhold, mouse-ear cress
Depositors F Lacroute
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pFL61
Intact vector size: 5.425
Type of vector: plasmid
Vector end: BstXI
Vector end: BstXI
Cloning sites: NotI
Polylinker sites: EcoRI BanII SacI Asp718 KpnI AvaI SmaI XmaI BamHI XbaI
Construction: pUC19
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, URA3
replicon: pMB1, 2 micron
Cross references:
Vector
DNA: cDNA
Insert size range: 0.3 - 3.0 kb
Genome Equivalent: 0.0
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.4 mL)
Comments
The inserts can be excised with a NotI digest.
Deposited as two cDNA banks of purified plasmid DNA.
Constructed by adding a BstXI adaptor 5'CTAAATTACTCACA-3'/3'-GATTTAATGA-5' to both ends of the cDNA.
Demonstrated to have an insert range of approximately 0.6 - 3.0 kb.
References

Minet M, et al. Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNA. Plant J. 2: 417-422, 1992. PubMed: 1303803

d'Enfert C, et al. . Methods Cell Biol. Plant Cell Biol. : submitted..

Francois Lacroute, personal communication

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