Charon BS(-) (ATCC® 77446)

Applications: expression vectorlambda vector allowing conversion to plasmidvector containing primer sites useful for sequencingvector for constructing cDNA librariesvector permitting production of single-stranded DNAvector permitting visual detection of recombinants  /  Depositors: A Swaroop

Permits and Restrictions

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Designations Charon BS(-)
Depositors A Swaroop
Biosafety Level 1
Vector Information
Size (kb): 43.1
Vector: Charon BS(-) (phage, lambda - replacement)
Promoters: Promoter for in vitro transcription T3
Construction: Charon 15, pBluescript KS M13(-)
Marker(s):ampR
Construct size (kb): 43.1
Features: insert detection: lacZ'
marker(s): ampR
other: left arm
other: pBluescript KS -
other: right arm
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: f1
replicon: pMB1
MCS: NotI...KpnI
MCS: SacI...NotI
Applications
expression vector
lambda vector allowing conversion to plasmid
vector containing primer sites useful for sequencing
vector for constructing cDNA libraries
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--31.0, 15.5, 6.0; EcoRI--25.0 (doublet); SalI--27.0, 13.5, 9.4; XhoI--27.0, 13.0, 9.2.
Vector for high-efficiency directional cloning. Clones can be converted to plasmids by recircularization after a NotI digest.
Charon BS(-) (ATCC 77446) and Charon BS(+) (ATCC 77447) differ in the orientation of the M13 origin of replication.
References

Swaroop A, Weissman SM. Charon BS(+) and (-), versatile lambda phage vectors for constructing directional cDNA libraries and their efficient transfer to plasmids. Nucleic Acids Res. 16: 8739, 1988. PubMed: 2971160

Shipped freeze-dried
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