7744 ™
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Vibrio fischeri (Beijerinck 1889) Lehmann and Neumann 1896 (Approved Lists 1980) and Photobacterium fischeri (Beijerinck 1889) Reichelt and Baumann 1973 (Approved Lists 1980) have the same type strain and therefore are homotypic synonyms (Rules 24a and 24b of the International Code of Nomenclature of Bacteria and Viruses).
This strain will grow on Marine Broth 2216 (BD 279110) and Marine Agar 2216 (BD 212185), but Photobacterium Medium is recommended for demonstrating luminescence. To check for luminescence, inoculate a slant, leaving cap loose. Incubate at 26oC for 18 hours. Then, hold in the dark for 10 minutes. If no luminescence is detected, reincubate and check again after 36, 48, and 72 hours. When maximum luminescence is obtained, stopper tightly.
This strain needs to establish good growth in broth culture before additional transfers are made. Additional incubation may be required for growth on solid media.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
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If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Hendrie MS, et al. The identification, taxonomy and classification of luminous bacteria. J. Gen. Microbiol. 64: 151-169, 1970.
Hendrie MS, et al. Proposal that Vibrio marinus (Russell 1891) Ford 1927 be amalgamated with Vibrio fischeri (Beijerinck 1889) Lehmann and Neumann 1896. Int. J. Syst. Bacteriol. 21: 217-221, 1971.
Eberhard A. Inhibition and activation of bacterial luciferase synthesis. J. Bacteriol. 109: 1101-1105, 1972. PubMed: 5011244
Sitnikov DM, et al. Control of cell division in Escherichia coli: regulation of transcription of ftsQA involves both rpoS and SdiA-mediated autoinduction. Proc. Natl. Acad. Sci. USA 93: 336-341, 1996. PubMed: 8552633
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.
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