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EY2 [lambdaLASEY2] (ATCC^® 77439^™)

Organism: Homo sapiens, human / Tissue: Library / Depositors: A Swaroop

EY2 [lambdaLASEY2] ATCC^® 77439^™

frozen 0.4 mL

For-Profit: $400.00 Non-Profit: $400.00

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Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Designations	EY2 [lambdaLASEY2]
Species	Homo sapiens, human
Depositors	A Swaroop
Origin	eye cell line
Vector	Vector :Charon BS- (ATCC 77446) Cloning capacity : < 8.9 kb Vector type : phage Intact vector size (kb) : 43.1 Construction : Charon 15, pBluescript KS M13(-) Features : (with orientation and position, when available) : Insert detection : lacZ', <-- Other : left arm : -->, 1-21225 Other : pBluescript KS - -->, 21226-24184 MCS : NotI?KpnI, --> , 21226-21315 Promoter for in vitro transcription : T3, <--, 21327-21346 Promoter : lac, <--, 21416-21444 Replicon : pMB1, 21771 Marker : ampR , <--, 22529-23389 Replicon : f1, -->, 23520-23976 Promoter for in vitro transcription : T7, -->, 24140-24159 MCS : SacI?NotI, -->, 24172-24184 Other : right arm, -->, 24185-43100
Vector	Tissue: eye, cell line CRL-7475 Type of insert: cDNA Insert size range: > 0.8 kb Genome Equivalent: 0.0 Genome: human Vector : Charon BS- Insert size range (kb) : > 0.8 kb Vector ends : Modification : EcoRI HindIII Number of independent recombinants : 1.0 x 10⁶ Titer : 1.3 x 10⁸ pfu/ml
Biosafety Level	1
Shipping Information	Frozen bacteria-free phage lysate.
Comments	cDNA synthesis was primed by oligo-dT, and a synthetic oligonucleotide (5'GCTTGAATTCAAGC 3') was added for directional cloning.
References	Swaroop A. Construction of directional cDNA libraries from human retinal tissue/cells and their enrichment for specific genes using an efficient subtraction procedure. Methods Neurosci. 15: 285-300, 1993. Swaroop A, Xu J. cDNA libraries from human tissues and cell lines. Cytogenet. Cell Genet. 64: 292-294, 1993. PubMed: 8404057 Swaroop A, Weissman SM. Charon BS(+) and (-), versatile lambda phage vectors for constructing directional cDNA libraries and their efficient transfer to plasmids. Nucleic Acids Res. 16: 8739, 1988. PubMed: 2971160

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Product Sheet	Product Sheet