EY2 [lambdaLASEY2] (ATCC® 77439)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: A Swaroop

Permits and Restrictions
Permits and Restrictions

View Permits

Designations EY2 [lambdaLASEY2]
Species Homo sapiens, human
Depositors A Swaroop
Origin
eye cell line
Vector
Vector :Charon BS- (ATCC 77446)
Cloning capacity : < 8.9 kb
Vector type : phage
Intact vector size (kb) : 43.1
Construction : Charon 15, pBluescript KS M13(-)
Features : (with orientation and position, when available) :
Insert detection : lacZ', <--
Other : left arm : -->, 1-21225
Other : pBluescript KS - -->, 21226-24184
MCS : NotI?KpnI, --> , 21226-21315
Promoter for in vitro transcription : T3, <--, 21327-21346
Promoter : lac, <--, 21416-21444
Replicon : pMB1, 21771
Marker : ampR , <--, 22529-23389
Replicon : f1, -->, 23520-23976
Promoter for in vitro transcription : T7, -->, 24140-24159
MCS : SacI?NotI, -->, 24172-24184
Other : right arm, -->, 24185-43100
Vector
Tissue: eye, cell line CRL-7475
Type of insert: cDNA
Insert size range: > 0.8 kb
Genome Equivalent: 0.0
Genome: human
Vector : Charon BS-
Insert size range (kb) : > 0.8 kb
Vector ends : Modification : EcoRI HindIII
Number of independent recombinants : 1.0 x 106
Titer : 1.3 x 108 pfu/ml
Biosafety Level 1
Shipping Information Frozen bacteria-free phage lysate.
Comments
cDNA synthesis was primed by oligo-dT, and a synthetic oligonucleotide (5'GCTTGAATTCAAGC 3') was added for directional cloning.
References

Swaroop A. Construction of directional cDNA libraries from human retinal tissue/cells and their enrichment for specific genes using an efficient subtraction procedure. Methods Neurosci. 15: 285-300, 1993.

Swaroop A, Xu J. cDNA libraries from human tissues and cell lines. Cytogenet. Cell Genet. 64: 292-294, 1993. PubMed: 8404057

Swaroop A, Weissman SM. Charon BS(+) and (-), versatile lambda phage vectors for constructing directional cDNA libraries and their efficient transfer to plasmids. Nucleic Acids Res. 16: 8739, 1988. PubMed: 2971160

Product Sheet
Product Sheet
Product Sheet